Size-exclusion chromatography of fractionated PC12 cells: enzyme activities of PPT1 and AGA. PC12 cells were homogenized and the 10 000g pellet of the postnuclear supernatant was isolated by centrifugation. Solubilised fraction was subjected to size exclusion chromatography and the PPT1 and AGA enzyme activities were measured from the fractions collected. The X-axis indicates time and the molecular weights are derived from the standard (Bio-Rad).
Lyly et al. BMC Cell Biology 2007 8:22 doi:10.1186/1471-2121-8-22