Figure 2.

Intracellular localization of wild type and glycosylation site mutated PPT1 in HeLa cells. HeLa cells were transfected with the wild type pCMV5-PPT1 plasmid or with plasmids carrying mutations S199A, S214A, S214A and S199A + S214A. The cells were double-stained using the polyclonal antibody for PPT1 (red) and the monoclonal antibody for LAMP-1, lysosomal membrane protein (green). Colocalization is shown in yellow.

Lyly et al. BMC Cell Biology 2007 8:22   doi:10.1186/1471-2121-8-22
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