Table 1

Phenotypes of myopathy-linked actin mutants expressed in C2C12 myoblasts and differentiated myotubes.

wt

H40Y

V163L

164N

N115S

G268R

D268R

G15R

und.

diff.

und.

diff.

und.

diff.

und.

diff.

und.

diff.

und.

diff.

und.

diff.

und.

diff.


n

189

219

174

222

203

189

182

128

52

89

135

127

140

110

114

108

cat. 1 %

74

57

44

41

25

40

15

55

52

67

65

72

14

35

58

42

cat. 2 %

26

3

56

36

75

26

85

5

48

5

35

8

86

44

42

30

aggr.

32

33

57

19

8

38

11

29

36

75

30

60

17

100

94

19

nucl.aggr.

0

0

31

0

56

38

0

0

0

0

0

0

0

0

0

0

deloc.

68

11

27

4

42

2

92

43

64

0

70

20

84

0

10

0

aberr. SF

0

67

0

91

0

68

0

57

0

25

0

20

0

12

0

88

cat. 3 %

33

5

7

23

22

17

7

11

cat. 4 %

7

18

25

17

2

2

11

18

aggr.

38

38

35

27

100

100

100

42

nucl.aggr.

0

21

58

0

0

0

0

0

deloc.

56

8

2

83

0

0

0

0

aberr. SF

13

49

40

0

0

0

8

84


Table 1 shows a summary of data from experiments where EGFP-tagged actin constructs were transfected into C2C12 cells and myoblasts were then differentiated for 4–6 days. After immunostaining, transfected myoblasts or differentiated myotubes were classified into categories depending on how well the EGFP-actin was integrated and the differentiation state of the cells:

myoblasts (und.): category 1: cells with good integration of EGFP-actin construct

category 2: cells with poor integration of EGFP-actin construct, divided into different subgroups: cytoplasmic aggregates (aggr.), nuclear aggregates (nucl. aggr.), delocalization (deloc.), aberrant stress fibres (aberr. SF)

myotubes (diff.): category 1: differentiated cells with good integration of EGFP-actin consructs

category 2: differentiated cells with poor integration (see subgroups above)

category 3: undifferentiated cells with good integration

category 4: undifferentiated cells with poor integration (see subgroups above)

For every construct, the total number of transfected cells that were counted is also stated (n).

Bathe et al. BMC Cell Biology 2007 8:2   doi:10.1186/1471-2121-8-2

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