Figure 3.

Expression of wild-type actin-EGFP. Wild-type muscle actin with a C-terminal EGFP-tag was expressed in undifferentiated C2C12 myoblasts (A) or myotubes that have been differentiated for 4–6 days (B, C). Expressed actin_EGFP co-localized with endogenous filamentous actin, as visualized with phalloidin in myoblasts (A, A") and myotubes (B, B"). In a subset of myotubes, a striated staining-pattern for alpha-actinin revealed sarcomeric structures, with expressed EGFP-actin co-localizing within the sarcomeric thin-filament (C, C"; inset). (Scale bars: 5 μm)

Bathe et al. BMC Cell Biology 2007 8:2   doi:10.1186/1471-2121-8-2
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