Figure 4.

DNA methylation analysis of adipogenic promoters in undifferentiated and differentiated ASCs at passage 4 (P4) and senescence (Ps). (A) Bisulfite sequencing of LEP, PPARG2, FABP4 and LPL in undifferentiated Ps cells. Numbers are as in Figure 3. (B) Percentage of individual methylated CpGs (● in A) in undifferentiated P4 (green) and Ps (red) cells (average of clones B1, B2, B3). Data for individual undifferentiated ASC clones are shown in Additional file 1, Supplementary Figure. (C) Bisulfite sequencing of Ps cells after adipogenic differentiation. Numbers are as in Figure 3. (D) Percentage of individual methylated CpGs (● in C) in differentiated cells at Ps. Data for individual differentiated clones are shown in Additional file 1, Supplementary Figure 2. **P < 0.01, ***P < 0.001 (t-tests).

Noer et al. BMC Cell Biology 2007 8:18   doi:10.1186/1471-2121-8-18
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