Table 1

Percent viability with tissue specific GAL4 drivers

vg- GAL4

twist- GAL4

scalloped- GAL4

eyeless- GAL4

collagen- GAL4


UAS-Bax-GFP

6.8

7.6

0

0

0

(BA64III)

(36/527)

(46/602)

(0/141)

(0/96)

(0/130)

UAS-Bax30–192-GFP

8.9

0.6

1.6

0

0

(DBA8II)

(55/616)

(2/315)

(1/59)

(0/120)

(0/137)

UAS-Bcl-2

90.4

104

97.5

(BC57I)

(377/417)

(259/248)

(39/40)


UAS transgenic lines for Bax-GFP (BA64III) or Bax 30–192-GFP (DBA8II) or Bcl-2 (BC57I) were screened for lethality using various tissue specific GAL4 lines as indicated. Percent viability was calculated by assessing the number of Bax-GFP or Bax 30–192-GFP or Bcl-2 expressing flies compared to the appropriate sibling control that eclosed at 25°C. Numbers in parenthesis indicate animals screened for analysis.

Parikh et al. BMC Cell Biology 2007 8:16   doi:10.1186/1471-2121-8-16

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