Western blot analysis of effect of PD98059 (PD), a MAPK pathway inhibitor on the phosphorylation of ERK1/2 in HDFs. Cells were incubated with PD (50 μM), thrombin (Th, 5 U/ml) or Th + PD at 37°C for 2 h or 6 h, respectively. Densitometry analysis of immunoblots was carried out using a Scion Image software. The relative levels of phospho-ERK1/2 were expressed as the ratio to β-actin. The values shown are Mean ± SD for four separate experiments. Cells from each one of the four dermal fibroblast donors were used for one independent experiment. † P < 0.05 compared with the response to medium alone (Con); * P < 0.05 compared with the response to thrombin alone.
Wang et al. BMC Cell Biology 2007 8:14 doi:10.1186/1471-2121-8-14