Western blot analysis of effect of AG490 (AG), a JAK/STAT pathway inhibitor on the phosphosrylation of STAT3 in HDFs. Cells were incubated with AG (40 μM), thrombin (Th, 5 U/ml) or Th + AG at 37°C for 30 min, 2 h and 6 h, respectively. Densitometry analysis of immunoblots was carried out using a Scion Image software. The relative levels of phospho-STAT3 were expressed as the ratio to β-actin. The values shown are Mean ± SD for four separate experiments. Cells from each one of the four dermal fibroblast donors were used for one independent experiment. † P < 0.05 compared with the response to medium alone (Con); * P < 0.05 compared with the response to thrombin alone.
Wang et al. BMC Cell Biology 2007 8:14 doi:10.1186/1471-2121-8-14