Real-time epifluorescence microscopy of calcein and ethidium homodimer fluorescence after treatment with 100 μM tBHP. DIC (left panels), calcein (right panels) and EH-1(middle panels) epifluorescence microscopy images, collected every two minutes after treatment with 100 μM of tBHP. Images are representative of still micrographs obtained from the time-lapse of cells treated with tBHP and labeled with calcein-AM and EH-1.
Sardão et al. BMC Cell Biology 2007 8:11 doi:10.1186/1471-2121-8-11