tBHP induced and increase intracellular oxidative stress in H9c2 cells. (Top panel) H9c2 cells were exposed to 0 (panel A-B) or 50 μM tBHP (panel C-D) for 1 hour. Increase in intracellular oxidative stress was detected by oxidation of dichlorofluorescein. Fluorescence observed in panel B and D is proportional to the degree of oxidation. The corresponding DIC images can be observed in panels A and C. Images are representative from 3 different cell preparations. A-D are the same magnification; scale bar in A = 20 μm. (Bottom panel) Quantification of cell DCF fluorescence intensity. Four different fields were analyzed in each experiment. Data represents the mean ± SEM of 3 different cell preparations. *p < 0.05 vs control.
Sardão et al. BMC Cell Biology 2007 8:11 doi:10.1186/1471-2121-8-11