Differential response of lymphatic, venous and arterial endothelial cells to angiopoietin-1 and angiopoietin-2
- Equal contributors
1 Molecular and Cellular Biology Research, Sunnybrook Research Institute, Sunnybrook Health Sciences Centre, Toronto, ON, Canada
2 Department of Medical Biophysics, University of Toronto, ON, Canada
3 Ontario Veterinary College, University of Guelph, Guelph, ON, Canada
4 Heart & Stroke/Richard Lewar Centre of Excellence, Faculty of Medicine, University of Toronto, Toronto, ON, Canada
5 R. Samuel McLaughlin Centre for Molecular Medicine, Toronto, ON, Canada
BMC Cell Biology 2007, 8:10 doi:10.1186/1471-2121-8-10Published: 6 March 2007
Additional File 1:
Cell counts of bmECs. Endothelial cells of each type (bmLEC, bmVEC, and bmAEC) were seeded in equal numbers (approximately 30–40% confluency) and counted every 24 hours. Trypan blue exclusion was used to determine cell viability. Numbers from three independent counts were used to compile the figure.
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Additional File 2:
Sequence alignment of bovine, murine, and human Tie-2 sequences. Amino acid sequences were obtained from Uniprot/Swiss-prot and aligned with CLUSTALW : Human Q02763, Bovine Q06807, Mouse Q02858. Boxed in blue are residues found to be important for Ang2 interaction with Tie-2 , all of which are conserved between the three species. The transmembrane domain is indicated by the black bar. All tyrosine residues in the cytoplasmic domain of Tie-2 are well conserved between the three species.
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Additional File 3:
Proliferative response of bmLECs to various growth factors. Compared to mock-treated bmLECs (anti-His clustering antibody), bmLECs treated with VEGF-A, VEGF-CCys156Ser, bFGF, and EGF, showed statistically significant increases in proliferation as displayed by 3H-thymidine uptake in 1% FBS. Results of two independent experiments were compiled for the figure. P-values from unpaired, two-tailed ttests for 95% CI are shown.
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