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Open Access Highly Accessed Research article

Differential response of lymphatic, venous and arterial endothelial cells to angiopoietin-1 and angiopoietin-2

Vicky PKH Nguyen12, Stephen H Chen12, Jason Trinh1, Harold Kim1, Brenda L Coomber3 and Daniel J Dumont1245*

Author Affiliations

1 Molecular and Cellular Biology Research, Sunnybrook Research Institute, Sunnybrook Health Sciences Centre, Toronto, ON, Canada

2 Department of Medical Biophysics, University of Toronto, ON, Canada

3 Ontario Veterinary College, University of Guelph, Guelph, ON, Canada

4 Heart & Stroke/Richard Lewar Centre of Excellence, Faculty of Medicine, University of Toronto, Toronto, ON, Canada

5 R. Samuel McLaughlin Centre for Molecular Medicine, Toronto, ON, Canada

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BMC Cell Biology 2007, 8:10  doi:10.1186/1471-2121-8-10

Published: 6 March 2007

Additional files

Additional File 1:

Cell counts of bmECs. Endothelial cells of each type (bmLEC, bmVEC, and bmAEC) were seeded in equal numbers (approximately 30–40% confluency) and counted every 24 hours. Trypan blue exclusion was used to determine cell viability. Numbers from three independent counts were used to compile the figure.

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Open Data

Additional File 2:

Sequence alignment of bovine, murine, and human Tie-2 sequences. Amino acid sequences were obtained from Uniprot/Swiss-prot and aligned with CLUSTALW [49]: Human Q02763, Bovine Q06807, Mouse Q02858. Boxed in blue are residues found to be important for Ang2 interaction with Tie-2 [18], all of which are conserved between the three species. The transmembrane domain is indicated by the black bar. All tyrosine residues in the cytoplasmic domain of Tie-2 are well conserved between the three species.

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Open Data

Additional File 3:

Proliferative response of bmLECs to various growth factors. Compared to mock-treated bmLECs (anti-His clustering antibody), bmLECs treated with VEGF-A, VEGF-CCys156Ser, bFGF, and EGF, showed statistically significant increases in proliferation as displayed by 3H-thymidine uptake in 1% FBS. Results of two independent experiments were compiled for the figure. P-values from unpaired, two-tailed ttests for 95% CI are shown.

Format: TIFF Size: 7.6MB Download file

Open Data