Table 4

Cell count and viability

Untreated HT1376 cells

6 hrs

16 hrs

24 hrs

48 hrs

72 hrs

96 hrs


Total cell count (106/mL)

5.625

9.375

10.42

17.3

23.625

27.25

% Viable cells

97.0

98.1

95.5

93.0

94.5

91.3

% Dead cells

3.0

1.9

4.5

7.0

5.5

8.7


DOC – treated HT1376 cells

6 hrs

16 hrs

24 hrs

48 hrs

72 hrs

96 hrs


Total cell count (106/mL)

4.281

6.887

7.706

10.545

9.647

8.557

% Viable cells

96.5

94.8

95.0

70.3

46.7

32.6

% Dead cells

3.5

5.2

5.0

29.7

53.3

67.4


HT 1376 bladder carcinoma cells were stained with Trypan Blue and evaluated with a hemocytometer, as described in Methods. Data reported in the table are from one representative experiment. Counting error was ± 0.15 × 106 cells. The count was repeated once, with virtually identical results.

Fabbri et al. BMC Cell Biology 2006 7:6   doi:10.1186/1471-2121-7-6

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