Table 3 |
|||||||||
|
Effect of pre-incubation with CsA on DOC-treated HT 1376 cells. |
|||||||||
|
1 h |
16 hrs |
24 hrs |
|||||||
|
|
|||||||||
|
Control |
|||||||||
|
|
|||||||||
|
JC-1 |
1.0 |
2.1 |
4.7 |
||||||
|
AnnV + PI staining |
E |
L |
N |
E |
L |
N |
E |
L |
N |
|
1.9 |
0.5 |
1.5 |
2.1 |
0.9 |
3.1 |
2.8 |
1.1 |
5.0 |
|
|
|
|||||||||
|
DOC-treated |
|||||||||
|
|
|||||||||
|
JC-1 |
1.0 |
9.5 |
24.0 |
||||||
|
AnnV + PI staining |
E |
L |
N |
E |
L |
N |
E |
L |
N |
|
2.1 |
0.9 |
2.0 |
5.0 |
0.5 |
3.5 |
6.0 |
2.5 |
5.5 |
|
|
|
|||||||||
|
CsA + DOC treated |
|||||||||
|
|
|||||||||
|
JC-1 |
1.0 |
5.2 |
23.1 |
||||||
|
AnnV + PI staining |
E |
L |
N |
E |
L |
N |
E |
L |
N |
|
2.0 |
0.7 |
2.5 |
5.0 |
1.1 |
4.2 |
5.0 |
4.3 |
8.4 |
|
|
|
|||||||||
|
HT 1376 bladder carcinoma cells were pre-incubated for 30 min with 1 μM CsA, after which 3 μg/mL docetaxel was added. After a 1-h treatment, both drugs were washed out and cells were further cultured. Data are percentages of cells with the indicated staining characteristics. For details, see the Methods section. JC-1 = cells undergoing Δψm transition; E = early apoptosis, i.e. AnnV+/PI-; L = late apoptosis, i.e. AnnV+/PI+; N = necrosis, i.e. AnnV-/PI+ |
|||||||||
|
Fabbri et al. BMC Cell Biology 2006 7:6 doi:10.1186/1471-2121-7-6 |
|||||||||
