Table 3

Effect of pre-incubation with CsA on DOC-treated HT 1376 cells.

1 h

16 hrs

24 hrs


Control


JC-1

1.0

2.1

4.7

AnnV + PI staining

E

L

N

E

L

N

E

L

N

1.9

0.5

1.5

2.1

0.9

3.1

2.8

1.1

5.0


DOC-treated


JC-1

1.0

9.5

24.0

AnnV + PI staining

E

L

N

E

L

N

E

L

N

2.1

0.9

2.0

5.0

0.5

3.5

6.0

2.5

5.5


CsA + DOC treated


JC-1

1.0

5.2

23.1

AnnV + PI staining

E

L

N

E

L

N

E

L

N

2.0

0.7

2.5

5.0

1.1

4.2

5.0

4.3

8.4


HT 1376 bladder carcinoma cells were pre-incubated for 30 min with 1 μM CsA, after which 3 μg/mL docetaxel was added. After a 1-h treatment, both drugs were washed out and cells were further cultured. Data are percentages of cells with the indicated staining characteristics. For details, see the Methods section.

JC-1 = cells undergoing Δψm transition; E = early apoptosis, i.e. AnnV+/PI-; L = late apoptosis, i.e. AnnV+/PI+; N = necrosis, i.e. AnnV-/PI+

Fabbri et al. BMC Cell Biology 2006 7:6   doi:10.1186/1471-2121-7-6

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