Table 2

Percentage of apoptotic cells: comparison of results obtained with different methods in the U937 myelomonocytic cell line

Method

Culture time


16 hrs

24 hrs

48 hrs

Untreated controls, 48 hrs


JC-1

15.6

27.3

56.4

4.0

MAA + PI staining

E

L

N

E

L

N

E

L

N

E

L

N

18.4

2.0

4.6

23.8

4.4

8.4

38.0

29.0

6.5

1.0

1.5

5.6

Ann V + PI staining

E

L

N

E

L

N

E

L

N

E

L

N

19.5

6.5

1.2

28.7

10.1

3.5

40.9

22.0

2.8

2.9

1.5

1.8


U937 myelomonocytic cells were treated for 1 hour with 3 μg/mL docetaxel, after which the drug was washed out and cells were further cultured. Data are the average of 2 experiments, with nearly identical results. For details, see the Methods section.

JC-1 = cells undergoing Δψm transition; E = early apoptosis, i.e. MAA+/PI- or AnnV+/PI-; L = late apoptosis, i.e. MAA+/PI+ or AnnV+/PI+; N = necrosis, i.e. MAA-/PI+ or AnnV-/PI+

Fabbri et al. BMC Cell Biology 2006 7:6   doi:10.1186/1471-2121-7-6

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