Table 3

Levels of Insulin Receptor Substrate Expression in SH-Sy5y Cells

Growth Factor

IRS-1/18S × 10-6

IRS-2/18S × 10-6

IRS-4/18S × 10-6


Control

7.18 ± 0.88*, +

1.19 ± 0.21

1.37 ± 0.27

Insulin

18.66 ± 2.21*, ++

2.18 ± 0.44 +

3.96 ± 0.61 +

IGF-1

32.61 ± 2.55*, ++

1.37 ± 0.24

2.01 ± 0.21 +


SH-Sy5y cells were stimulated with vehicle (Control), 50 nM insulin, or 25 nM IGF-1 for 24 hours, then analyzed by real time quantitative RT-PCR to measure mRNA levels of insulin receptor substrate (IRS) subtypes 1, 2, and 4 using the primer pairs listed in Table 2. The results were normalized to 18S ribosomal RNA measured in the same samples. The values listed represent mean ± S.D. of results obtained from 6 replicate cultures. *P < 0.001 relative to IRS-2 and IRS-4 measured in the same cultures. ++ P < 0.001; + P < 0.05 relative to control.

Lahousse et al. BMC Cell Biology 2006 7:41   doi:10.1186/1471-2121-7-41

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