Comparison of AAH (A), Humbug (B), and Junctin (C) mRNA levels in SH-Sy5y, CNS-derived PNET1 and PNET2 primitive neuroblastic cells, normal human infant frontal cortex, and normal human adult frontal cortex. RNA was reverse transcribed with random oligodeoxynucleotide primers, and the cDNA templates were used to measure gene expression by real time quantitative PCR. The results were normalized to 18S measured in the same samples. The graphs depict the mean ± S.D. of results obtained from 6 replicate independent cultures, 2 infant brains, and 6 adult brains. Inter-group comparisons were made using ANOVA with post-hoc Fisher Least Significant Difference (LSD) tests (+ P < 0.05; * P < 0.001 relative to Sh-Sy5y cells in A and B, and relative to PNET1 cells in C).
Lahousse et al. BMC Cell Biology 2006 7:41 doi:10.1186/1471-2121-7-41