(a) β-galactosidase staining of normal skin from TOPGAL mice, which express the β-galactosidase reporter gene under the control of a promoter responsive to β-catenin-dependent signaling shows little to no β-galactosidase activity. (b) β-galactosidase staining 7 days after wounding shows activity present in the differentiated hair follicle shafts immediately adjacent to the wound edge (arrow). The actual wound is to the right of the arrow. (c) The staining for β-galactosidase was most intense 21 days post wounding, with the wound being to the right of the arrow. (d) At no time point was β-gal activity detected within the wound itself (a representative 21 day wound is shown).
Fathke et al. BMC Cell Biology 2006 7:4 doi:10.1186/1471-2121-7-4