Figure 2.

Validation and extension of microarray analysis by quantitative/real-time PCR. Primer sets (see 1) were designed to target the Affymetrix probe set region of the selected genes and to amplify the region across exon-exon junctions whenever possible. Quantitative PCR was performed as described in Materials and Methods. Shown is the mean of all signal values obtained from 6 independent RNA samples. (A) novel NETs; (B) NE markers proteins; (C) muscle differentiation markers. The error bars indicate the standard deviation. Gray columns indicate the fold changes obtained in the microarray analysis, taken from Fig. 1.