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Open Access Highly Accessed Research article

Nuclear envelope transmembrane proteins (NETs) that are up-regulated during myogenesis

I-Hsiung Brandon Chen, Michael Huber, Tinglu Guan, Anja Bubeck and Larry Gerace*

Author Affiliations

Department of Cell Biology, The Scripps Research Institute, 10555 N. Torrey Pines Rd., La Jolla CA 92037, USA

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BMC Cell Biology 2006, 7:38  doi:10.1186/1471-2121-7-38

Published: 24 October 2006

Additional files

Additional File 1:

NETs and control genes examined by microarray and RT-PCR analysis. List of all genes analyzed in this study. This includes an updated list of NETs originally described in Ref. [22] together with gene annotations from standard BLAST analysis, a list of primers used for quantitative RT-PCR, and the probesets on Affymetrix Mouse Genome 430 2.0™ array that were recognized by the specific genes indicated

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Additional File 2:

Relative levels of NE markers and desmin at three developmental stages in 10 mouse tissues. Gene expression levels of selected lamina proteins were measured by quantitative real-time PCR in ten C129 mouse tissues from embryonic day 17 (E7), post-natal day 7 (P7) and adult. All signal values were normalized to peptidylprolyl isomerase A (Ppia) as in Fig. 4. The Y-axis indicates the signal value of RNA transcript after normalization to Ppia. The error bars indicate the standard deviation.

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Additional File 3:

Relative levels of NETs up-regulated in C2C12 differentiation at three developmental stages in 10 mouse tissues. Gene expression levels of the 6 NETs up-regulated during C2C12 differentiation were measured by quantitative real-time PCR in ten C129 mouse tissues from embryonic day 17 (E7), post-natal day 7 (P7) and adult. All signal values were normalized to peptidylprolyl isomerase A (Ppia) as in Fig. 4. The Y-axis indicates the signal value of RNA transcript after normalization to Ppia. The error bars indicate the standard deviation.

Format: TIFF Size: 5.4MB Download file

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Additional File 4:

Expression range of selected lamina proteins and the up-regulated NETs. A box-and-whisker graph shows gene expression levels of selected lamina proteins and the NETs in all tested mouse tissues. The expression values measured by RT-PCR in 2 and 3 were plotted on a log base 10 scale. Boxes indicate the median, the lower quartile and upper quartile values. Whiskers indicate the range of data points from the highest to the lowest. See [63].

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