Nanoelectropulse-induced phospholipid rearrangement and PS externalization does not require calcium. FM1-43 fluorescence intensification was photometrically measured 5 min after exposure to 20, 3 ns, 6.0 MV/m pulses. Experimental samples had either 5 mM EGTA in the medium (added immediately before pulse delivery) or were loaded with 10 μM BAPTA-AM for 60 min before pulsing.
Vernier et al. BMC Cell Biology 2006 7:37 doi:10.1186/1471-2121-7-37