Circumferential variation of membrane perturbation and PS externalization after pulse exposure. Pulse-induced changes in FM1-43 fluorescence intensity after delivery of 4, 30 ns, 2.5 MV/m pulses were extracted photometrically from microscope images by defining tangential regions of interest approximately 2 μm by 0.5 μm at 0°, 30°, 60°, 90°, 120°, 150°, and 180° around the circumference of a Jurkat T cell image, with 0° at the anode pole. This data was extracted from the results summarized in Fig. 4 of  to demonstrate for a single cell both the kinetics of the FM1-43 fluorescence intensification after pulse exposure, and the angular dependence of the response.
Vernier et al. BMC Cell Biology 2006 7:37 doi:10.1186/1471-2121-7-37