Figure 6.

The long-term activation of the p70S6 kinase pathway in HC11 cells. A: HC11 cells were induced to differentiate in DIP-induction media with and without EGF (10 μg/ml) and LY294002 (10 μM) for times up to 24 hours. Lysates were harvested and analyzed by western blotting using antibodies specific for phosphorylated p70S6 kinase B: HC11 cells were exposed to dexamethasone (1 μM) with and without EGF (10 μM) and LY294002 (10 μM) for times up to 24 hours. Lysates were harvested and analyzed by western blotting using antibodies specific for phosphorylated p70S6 kinase or actin. The arrows indicate the position of the phospho-p70S6 kinase band.

Galbaugh et al. BMC Cell Biology 2006 7:34   doi:10.1186/1471-2121-7-34
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