Additional file 2.
The appearance of a stimulated compared to an unstimulated cell, high power. An NIH3T3 culture was serum deprived, injected with the PH-AKT-GFP plasmid, and stimulated as above. Fluorescent confocal images (63X/1.4 objective) beginning at the coverslip and extending through the cell were taken of one cell 10 min following serum stimulation, and of another cell in an unstimulated culture prepared in parallel. The membrane structures observable following stimulation can be compared to the cytoplasmic localization in the unstimulated cell.
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Yang et al. BMC Cell Biology 2006 7:33 doi:10.1186/1471-2121-7-33