The rad26Δ and rad24Δ alleles may cause TBZ-sensitivity by a pathway that is independent of the cytokinesis checkpoint. A. The rad26Δ rad24Δ strain did not display an additive phenotype on TBZ medium. Cultures of wild type (TE236), rad26Δ (TE257), rad24Δ (TE465) and rad26Δ rad24Δ (TW1235) were serially diluted and manually spotted onto YE5S and YE5S + 8, 14 and 16 μg/ml TBZ. Pictures were taken after 3 days of growth at 30°C. B. rad24Δ, but not rad26Δ, was sensitive to LatA. Cultures were spotted onto YE5S plates + 0.5 μM LatA. C. The cytokinesis checkpoint of rad26Δ cells was intact. Liquid YE5S cultures of wild type (TE236), rad26Δ (TE257) and rad24Δ (TE465) were left untreated or treated with 0.2 μM LatA for 5 hours, fixed with paraformaldehyde and stained with DAPI.
Baschal et al. BMC Cell Biology 2006 7:32 doi:10.1186/1471-2121-7-32