A chart of the optimized protocols to isolate CD34+/-, CD133+/- and Lin-/+ cells from cord blood. Isolation of CD34/- and CD133+/- cells was performed using Direct CD34 Progenitor Cell Isolation Kit (#130-046-702, Miltenyi Biotec) and CD133 Cell Isolation Kit (#130-050-801, Miltenyi Biotec), respectively. Lin-/+ cells were isolated using StemSep Human Progenitor Enrichment Kit (#14056, StemCell Technologies). For all magnetic separations, MACS columns and separators (Miltenyi Biotech) were used. Abbreviations: MNC, mononuclear cells; Buffer, PBS pH 7.2 supplemented with 0.5% bovine serum albumin and 2 mM EDTA or 0.6% ACD/A.
Kekarainen et al. BMC Cell Biology 2006 7:30 doi:10.1186/1471-2121-7-30