Table 1

Analysis of the morphological defects and mitotic progression of mitosis in live images of RNAi transfected cells vs. antibody-injected cells.

Measurement

Control RNAi

MCAK RNAi

Control IgG

Anti-MCAK


Alignment Defects

% cells (n)

% cells (n)

% cells (n)

% cells (n)

Chromosomes lingering at poles

26 (17)

51 (15)

18 (17)

38 (16)

Multiple attempts to congress

33 (17)

49 (15)

18 (17)

56 (16)

Not all chromosomes congress

4 (16)

13 (13)

6 (17)

27 (16)

Loose alignment with high oscillations

13 (16)

89 (16)

24 (17)

87 (16)

Total % cells with alignment defects

39 (18)

88 (16)

41 (17)

88 (17)

Segregation Defects

% cells (n)

% cells (n)

% cells (n)

% cells (n)

Straggling chromosomes

11 (17)

53 (12)

25 (16)

50 (16)

Lagging chromosomes

0 (17)

6 (12)

6 (16)

23 (13)

Total % cells with segregation defects

12 (17)

42 (12)

25 (16)

50 (16)


Measurement

Control RNAi

MCAK RNAi

Control IgG

Anti-MCAK


Spindle Defects

Avg. ± SEM (n)

Avg. ± SEM (n)

Avg. ± SEM (n)

Avg. ± SEM (n)

Spindle length (μm)

15.3 ± 0.5 (14)

12.9 ± 0.5* (12)

14.5 ± 0.6 (16)

12.9 ± 0.4* (17)


Timing of Mitotic Progression

Avg. ± SEM (n)

Avg. ± SEM (n)

Avg. ± SEM (n)

Avg. ± SEM (n)

Time between injection and NEB (min)

NA

NA

5.6 ± 2.2 (17)

2.8 ± 0.8 (18)

Time between NEB and LCC (min)

17.4 ± 1.3 (14)

23.3 ± 7.0 (12)

16.1 ± 1.7 (15)

32.3 ± 3.4* (17)

Time between LCC and Ana A (min)

13.7 ± 2.7 (13)

12.6 ± 1.8 (11)

16.1 ± 1.7 (14)

17.4 ± 2.2 (18)

Total time between NEB and Ana A (min)

30.1 ± 2.7 (17)

32.6 ± 5.9 (14)

33.3 ± 1.7 (17)

47.7 ± 4.1* (18)


NEB, Nuclear envelope breakdown, LCC, the time when the last chromosome begins congressing. NA, not applicable. * Represents a p-value of <0.05 and indicates a significant difference in spindle length or timing.

Stout et al. BMC Cell Biology 2006 7:26   doi:10.1186/1471-2121-7-26

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