P-Eg5 and H-Eg5 share significant identity in their catalytic domains. A series of degenerate oligonucleotides were used to clone a segment of both the H-Eg5 and P-Eg5 cDNA by RT-PCR. (A) An alignment of P-Eg5 and H-Eg5 DNA is shown. The sequence chosen for the 21 bp siRNA is outlined in green.
Stout et al. BMC Cell Biology 2006 7:26 doi:10.1186/1471-2121-7-26