Additional File 2.


The movies are viewable using QuickTime, which can be downloaded from Apple's QuickTime Site webcite. 3D-image reconstructions of A) cytoplasmically injected fluorescent actin and B) CD-BODIPY visualised in living HeLa cells expressing fluorescent NPC (POM121-GFP). M1B shows a maximum-intensity projection three-dimensional rendering of a confocal stack recorded from an H-PomGFP cell labelled with 50 nM CD-BODIPY. CD-BODIPY channel is pseudo-colored red, whereas POM121-GFP is pseudo-colored green. Note in both movies actin fluorescence distribution around the NE-membrane is punctate.

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M√ľnter et al. BMC Cell Biology 2006 7:23   doi:10.1186/1471-2121-7-23