Research article
Actin polymerisation at the cytoplasmic face of eukaryotic nuclei
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* Corresponding author: Spencer L Shorte sshorte@pasteur.fr
1 Unité de Biologie Cellulaire du Noyau, CNRS URA 2582, Département de Biologie Cellulaire et Infection, Institut Pasteur; 25, Rue du Docteur Roux, 75724 Paris Cedex 15, France
2 Current address: Department of Parasitology, Heidelberg University School of Medicine, Im Neuenheimer Feld 326, 69120 Heidelberg, Germany
3 Unité de Pathogénie Microbienne Moléculaire, U389 INSERM Institut Pasteur; 28, Rue du Docteur Roux, 75724 Paris Cedex 15, France
4 Unité de Embryologie Moléculaire, Institut Pasteur; 25, Rue du Docteur Roux, 75724 Paris Cedex 15, France
5 Current address: Department of Cell Biology, University of Cordoba, 14014-Cordoba, Spain
6 Département de Biologie Cellulaire, Institut Jacques Monod, CNRS, Universités Paris 6 and 7, 75251 Paris Cedex 05, France
7 Plate-Forme d'Imagerie Dynamique (PFID), Département de Biologie Cellulaire et Infection, Institut Pasteur; 25, Rue du Docteur Roux, 75724 Paris Cedex 15, France
BMC Cell Biology 2006, 7:23 doi:10.1186/1471-2121-7-23
Published: 23 May 2006Additional files
The movies are viewable using QuickTime, which can be downloaded from Apple's QuickTime Site http://www.apple.com/quicktime/download/ webcite. 3D-image reconstructions of A) cytoplasmically injected fluorescent actin and B) CD-BODIPY visualised in living HeLa cells expressing fluorescent NPC (POM121-GFP). M1A shows a maximum-intensity projection of three-dimensional dataset rendered from a confocal stack recording of an H-PomGFP cell subjected to cytoplasmic microinjection of fluorescent actin just minutes before the experiment. In this overlay movie projection the actin channel is pseudo-colored red, whereas POM121-GFP is pseudo-colored green.
Format: MOV Size: 1.2MB Download file
Playing the movie within this page requires QuickTime and JavaScript. Read more
The movies are viewable using QuickTime, which can be downloaded from Apple's QuickTime Site http://www.apple.com/quicktime/download/ webcite. 3D-image reconstructions of A) cytoplasmically injected fluorescent actin and B) CD-BODIPY visualised in living HeLa cells expressing fluorescent NPC (POM121-GFP). M1B shows a maximum-intensity projection three-dimensional rendering of a confocal stack recorded from an H-PomGFP cell labelled with 50 nM CD-BODIPY. CD-BODIPY channel is pseudo-colored red, whereas POM121-GFP is pseudo-colored green. Note in both movies actin fluorescence distribution around the NE-membrane is punctate.
Format: MOV Size: 1.1MB Download file
Playing the movie within this page requires QuickTime and JavaScript. Read more