Open Access Research article

Actin polymerisation at the cytoplasmic face of eukaryotic nuclei

Sylvia Münter12, Jost Enninga3, Rafael Vazquez-Martinez45, Erwan Delbarre6, Brigitte David-Watine1, Ulf Nehrbass1 and Spencer L Shorte7*

Author Affiliations

1 Unité de Biologie Cellulaire du Noyau, CNRS URA 2582, Département de Biologie Cellulaire et Infection, Institut Pasteur; 25, Rue du Docteur Roux, 75724 Paris Cedex 15, France

2 Current address: Department of Parasitology, Heidelberg University School of Medicine, Im Neuenheimer Feld 326, 69120 Heidelberg, Germany

3 Unité de Pathogénie Microbienne Moléculaire, U389 INSERM Institut Pasteur; 28, Rue du Docteur Roux, 75724 Paris Cedex 15, France

4 Unité de Embryologie Moléculaire, Institut Pasteur; 25, Rue du Docteur Roux, 75724 Paris Cedex 15, France

5 Current address: Department of Cell Biology, University of Cordoba, 14014-Cordoba, Spain

6 Département de Biologie Cellulaire, Institut Jacques Monod, CNRS, Universités Paris 6 and 7, 75251 Paris Cedex 05, France

7 Plate-Forme d'Imagerie Dynamique (PFID), Département de Biologie Cellulaire et Infection, Institut Pasteur; 25, Rue du Docteur Roux, 75724 Paris Cedex 15, France

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BMC Cell Biology 2006, 7:23  doi:10.1186/1471-2121-7-23

Published: 23 May 2006

Additional files

Additional File 1:

The movies are viewable using QuickTime, which can be downloaded from Apple's QuickTime Site http://www.apple.com/quicktime/download/ webcite. 3D-image reconstructions of A) cytoplasmically injected fluorescent actin and B) CD-BODIPY visualised in living HeLa cells expressing fluorescent NPC (POM121-GFP). M1A shows a maximum-intensity projection of three-dimensional dataset rendered from a confocal stack recording of an H-PomGFP cell subjected to cytoplasmic microinjection of fluorescent actin just minutes before the experiment. In this overlay movie projection the actin channel is pseudo-colored red, whereas POM121-GFP is pseudo-colored green.

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Open Data

Additional File 2:

The movies are viewable using QuickTime, which can be downloaded from Apple's QuickTime Site http://www.apple.com/quicktime/download/ webcite. 3D-image reconstructions of A) cytoplasmically injected fluorescent actin and B) CD-BODIPY visualised in living HeLa cells expressing fluorescent NPC (POM121-GFP). M1B shows a maximum-intensity projection three-dimensional rendering of a confocal stack recorded from an H-PomGFP cell labelled with 50 nM CD-BODIPY. CD-BODIPY channel is pseudo-colored red, whereas POM121-GFP is pseudo-colored green. Note in both movies actin fluorescence distribution around the NE-membrane is punctate.

Format: MOV Size: 1.1MB Download file

Playing the movie within this page requires QuickTime and JavaScript. Read more

Open Data