Table 4

Effect of the inhibitors of tryptase or elastase on tryptase or elastase induced release of IL-8 from A549 cells

Compound (μg/ml)
IL-8 released (pg/ml)

Medium alone
24.6 ± 1.5
Tryptase 2.0
105.2 ± 5.3*
Elastase 0.3
108.4 ± 13.2*
Benzamidine 30
37.6 ± 4.4
Leupeptine 30
27.4 ± 5.6
MSACK 30
22.1 ± 4.3
Tryptase 2.0 + Benzamidine 30
66.9 ± 4.8
Tryptase 2.0 + Leupeptin 30
100 ± 1.7
Elastase 0.3 + MSACK 30
26.9 ± 3.6

Data shown are mean ± SE for five to six separate experiments. Stimulus, inhibitor, or stimulus with inhibitor was incubated with cells for 16 h. * P < 0.05 compared with the response to medium alone; P < 0.05 compared with the corresponding stimulus alone.

Wang et al. BMC Cell Biology 2006 7:22   doi:10.1186/1471-2121-7-22