Time course of hTERT, c-myc and PPARγ mRNA expression after treatment of normal human PBMCs with growth medium containing 10% NHS and PMA (100 nM). Total cellular RNA was extracted at indicated time points (h-hours) and analyzed by RT-PCR. (A) Representative agarose gel photographs showing ethidium bromide stained RT-PCR products of hTERT, c-myc, PPARγ and β2M genes. (B) The signal intensities of these RT-PCR products were quantified using SCION IMAGE analysis software. The relative levels of hTERT, c-myc and PPARγ mRNA expression were obtained by the ratio of their individual band intensity to β2M band intensity. The normalized mRNA expression of each gene at 4, 8 and 12 h is plotted as percentage of that in control cells (0 h). Each data point in the graph represents mean ± SD of three separate experiments.
Sikand et al. BMC Cell Biology 2006 7:2 doi:10.1186/1471-2121-7-2