Effect of PPARγ silencing on hTERT gene transcription. Normal human PBMCs were transfected with scrambled siRNA (control scram siRNA) or with siRNA targeting PPARγ mRNA (PPARγ siRNA). These cells were cultured for 72 hours and then subjected to RNA extraction and RT-PCR analysis. (A) Representative agarose gel photographs showing ethidium bromide stained RT-PCR products of PPARγ, hTERT and β2M genes. (B) The signal intensities of RT-PCR products were measured using SCION IMAGE analysis software. The mRNA expression was determined by normalizing the band intensity of target mRNA (hTERT and PPARγ) to β2M band intensity. The hTERT and PPARγ mRNA levels in cells transfected with PPARγ siRNA are expressed as percentage of that in control (scram siRNA) cells. Each bar represents mean ± SD of experiments done in triplicate.
Sikand et al. BMC Cell Biology 2006 7:2 doi:10.1186/1471-2121-7-2