The mRNA expression of hTERT, c-myc and PPARγ genes in normal human PBMCs treated with growth medium containing 10% NHS along with antibody against Receptor Ck (Ab RCk). Total cellular RNA was extracted at indicated time points (h-hours) and analyzed by RT-PCR. (A) Representative agarose gel photographs showing ethidium bromide stained RT-PCR products of hTERT, c-myc, PPARγ and β2M genes. (B) The signal intensities of these RT-PCR products were measured using SCION IMAGE analysis software. The mRNA expression was determined by normalizing the band intensity of target mRNA (hTERT, c-myc and PPARγ) to β2M band intensity. Data are expressed as percentage of control (cells harvested at 0 h). Each data point in the graph represents mean ± SD of three independent experiments.
Sikand et al. BMC Cell Biology 2006 7:2 doi:10.1186/1471-2121-7-2