Time course of hTERT, c-myc and PPARγ mRNA expression in normal human PBMCs exposed to growth medium enriched with 10% NHS. The cells were harvested at indicated time points (h-hours) and subjected to RNA extraction followed by RT-PCR. (A) Representative agarose gel photographs showing ethidium bromide stained RT-PCR products of hTERT, c-myc, PPARγ and β2M genes. (B) The signal intensities of RT-PCR products shown in panel A were measured using SCION IMAGE analysis software. The relative levels of hTERT, c-myc and PPARγ mRNA expression in each lane were determined by normalizing their individual band intensity to β2M band intensity. The mRNA expression of each gene at 4, 8 and 12 h is plotted as percentage of that in control cells (i.e. cells harvested at 0 h). Each data point in the graph represents mean ± standard deviation (SD) of three separate experiments.
Sikand et al. BMC Cell Biology 2006 7:2 doi:10.1186/1471-2121-7-2