Thalidomide reduces the sub-population of the SNP treated cells with higher membrane actin polymerization pattern: Analysis of the images obtained from phalloidin experiments gives two sub-populations with varying fluorescent intensity of phalloidin 1) low intensity membrane pattern of actin polymerization described as Low AP and 2) Higher intensity of actin polymerization in the plasma membrane described as High AP (upper panel). SNP treatments of ECs caused an increase in the number of high AP cells. Further treatments of these cells with three concentrations of thalidomide (25, 50 and 75 μg/ml) reduced the number of high AP cells in a dose dependent manner. Particularly, 50 and 75 μg/ml groups significantly reduced the high AP population with a corresponding increase in low AP cells. SNP, Tx25, Tx50 and Tx75 denote 500 μmol SNP, 25, 50 and 75 μg/ml thalidomide respectively. The asterisks represent the level of significance. * P < 0.05 compared to only SNP treated cells.
Tamilarasan et al. BMC Cell Biology 2006 7:17 doi:10.1186/1471-2121-7-17