Experimental design for analyzing multi-dimensional dynamics of differentiation. Shaded block arrows represent cells exposed to 0.8% DMSO for the indicated durations while open arrows indicate "untreated" cell cultures. Brackets indicate subpopulations sorted with FACS. The fraction of CD11bHigh cells was monitored by flow cytometry daily for 7 days for the "restimulated", "native" (mock-sorted and restimulated) and "untreated" groups. A CD11bLow fraction that was re-established upon restimulation with 0.8% DMSO for 4 days was also sorted, restimulated a second time ("twice restimulated"), and monitored by flow cytometry for 6 days.
Chang et al. BMC Cell Biology 2006 7:11 doi:10.1186/1471-2121-7-11