Figure 3.

Inhibiting GSK3β with lithium chloride enhances Notch signals in primary human CD4+, but not Jurkat, T-cells. (A) Primary human CD4+ T-cells were nucleofected with 4 μg p10xCBF-luc, 0.5 μg pTK-RL and 1 μg of either pN1-IC or pcDNA3.1 and plated o/n in triplicate with increasing concentrations of LiCl. Data is presented as the fold induction in normalised luminescence compared to the untreated vector only control. (B) Primary human CD4+ T-cells were nucleofected with 4 μg p10xCBF-luc and exposed to different levels of plate-bound Delta1-Fc in the presence or absence of LiCl (10 mM). (C) J-N2 cells were transfected with p10xCBF1-luc and pTK-RL and stimulated overnight in increasing concentrations of LiCl. Data is presented as the fold induction in normalised luminescence compared to the untreated IgG4 control.

Mckenzie et al. BMC Cell Biology 2006 7:10   doi:10.1186/1471-2121-7-10
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