Additional File 4.
Chromosomes of prophase nucleus of an embryo exposed to normoxia. Embryos were collected, and stained with DAPI, mAb414 to recognize nuclear pore complex and anti-gamma tubulin to recognize the centrosome. Spinning disk confocal microscopy was used to obtain Z-stack images of the prophase nucleus. The movie (QuickTime Player 7.0.3) depicts a 10.0 μm region that includes the nucleus cross-sectioned every 0.2 μm for a total of 50 cross sections. DAPI, mAb414 and anti-gamma tubulin images were merged to evaluate prophase chromosome location relative to the nuclear membrane.
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Hajeri et al. BMC Cell Biology 2005 6:47 doi:10.1186/1471-2121-6-47