GFP-CpnA labels contractile vacuoles. Cells expressing GFP-CpnA were placed in water for 1.5 minutes, fixed, and imaged using differential interference contrast microscopy (A, C). These same cells were imaged for GFP with a widefield fluorescence microscope (B, D). Contractile vacuoles in cells expressing GFP-CpnA were labeled with a primary antibody to calmodulin and a TRITC-conjugated secondary antibody and then imaged for TRITC (E, G, I). These same cells were imaged for GFP (F, H, J). Arrowheads point to contractile vacuoles.
Damer et al. BMC Cell Biology 2005 6:46 doi:10.1186/1471-2121-6-46