Figure 5.

GFP-CpnA associates with the plasma membrane and intracellular vacuoles in live disrupted cells in a calcium-dependent manner. Cells expressing GFP-CpnA were placed in water (A) or water with 2 mM EGTA (B) and scanned every 2.5 seconds on a confocal microscope. C) Cells expressing GFP only were placed in water and scanned every 2.5 seconds on a confocal microscope. Images shown are time-lapse sequences of cells in which the plasma membrane has been disrupted. See Additional Files – Movies 6, 7, 8.