Figure 5.

GFP-CpnA associates with the plasma membrane and intracellular vacuoles in live disrupted cells in a calcium-dependent manner. Cells expressing GFP-CpnA were placed in water (A) or water with 2 mM EGTA (B) and scanned every 2.5 seconds on a confocal microscope. C) Cells expressing GFP only were placed in water and scanned every 2.5 seconds on a confocal microscope. Images shown are time-lapse sequences of cells in which the plasma membrane has been disrupted. See Additional Files – Movies 6, 7, 8.

Damer et al. BMC Cell Biology 2005 6:46   doi:10.1186/1471-2121-6-46
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