GTPγS and 2'-deoxy-cAMP induced adenylyl cyclase activation. AX2 or HSB61 cells were pulsed with cAMP for 5 or 7 hours, respectively. Cell lysates were prepared in the presence of (grey bars) GTPγS, (black bars) 2'-deoxy-cAMP, (black stripes) MnCl2 and assayed for adenylyl cyclase activity. Plotted values were normalized relative to the (open bars) unstimulated activity obtained in the absence of GTPγS or 2'-deoxy-cAMP (0.7 pmol/mg/min and 0.6 pmol/mg/min for AX2 and HSB61 respectively). Values for AX2 and HSB61 cell lysates are the means ± sd of two indipendent experiments run in duplicate.
Arigoni et al. BMC Cell Biology 2005 6:43 doi:10.1186/1471-2121-6-43