Cyclic AMP accumulation in AX2 and HSB61 during development in shaken suspension. (Squares) AX2 or (triangles) HSB61 cells were incubated in suspension for the time indicated in the abscissa. (A) control cells or (B) cells treated with cAMP pulses every 6 minutes. At the time indicated, cell aliquots were quenched with perchloric acid, neutralised with KOH, and total cAMP in the samples was determined by radioimmunoassay as described in Material and Methods.
Arigoni et al. BMC Cell Biology 2005 6:43 doi:10.1186/1471-2121-6-43