Actin polymerization assay in response to chemoattractant stimulation. The cells concentrated at 2 × 107/ml were stimulated with 1.0 μm cAMP (time 0) and the F-actin formation measured with the phalloidin binding assay as described in Material and Methods. Starving (A) AX2 and (B, C) HSB61 cells were treated with cAMP pulses and tested for F-actin polymerization after (A, B) 4 or (C) 6 hours of starvation.
Arigoni et al. BMC Cell Biology 2005 6:43 doi:10.1186/1471-2121-6-43