Figure 14.

Actin polymerization assay in response to chemoattractant stimulation. The cells concentrated at 2 × 107/ml were stimulated with 1.0 μm cAMP (time 0) and the F-actin formation measured with the phalloidin binding assay as described in Material and Methods. Starving (A) AX2 and (B, C) HSB61 cells were treated with cAMP pulses and tested for F-actin polymerization after (A, B) 4 or (C) 6 hours of starvation.

Arigoni et al. BMC Cell Biology 2005 6:43   doi:10.1186/1471-2121-6-43
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