RasC and RasG activation in HSB61 mutant. AX2 or HSB61 cells were pulsed with cAMP for the time indicated, concentrated ten times, treated with cAMP and immediately lysed. The lysates were incubated with GST-Byr2 (RBD) as described in Material and Methods. The precipitate was subjected to electrophoresis and Western blot and hybridized with antibodies against RasC or RasG. Time (in seconds) after cAMP treatment is indicated. Total RasC and RasG in cell lysates from AX2 or HSB61 are shown on the right.
Arigoni et al. BMC Cell Biology 2005 6:43 doi:10.1186/1471-2121-6-43