Gene expession analysis in bovine intestinal epithelium, organoids and cultured cells. RT-PCR analysis of several gene expression in bovine intestinal epithelia, isolated organoids and cultured cells. The fragment size (bp) of amplified cDNA fragment was determined after migration on agarose (1%) gels and subsequent staining with ethidium bromide. Primers directed against (A) β-actin, amplicon size 485 bp, used as a quality control of cDNA obtained by reverse transcription. Amplifications of epithelial and enterocyte specific markers were obtained using primers directed against (B) FABP, 568 bp, (C) villin, 384 bp, (D) ZO1, 272 bp, (E) small intestinal peptidase, 276 bp, (F) vimentin, 548 bp and (G) E-cadherin, 308 bp. Lanes: 1, freshly scrapped colon epithelium; 2, organoid suspension from colon; 3, primary culture of colonocytes; 4, cultured colonocytes after the first passage; 5, freshly scrapped jejunum epithelium; 6, organoid suspension from jejunum; 7, primary culture of jejunocytes; 8, cultured jejunocytes after the first passage; 9, molecular weight standard in bp.
Rusu et al. BMC Cell Biology 2005 6:42 doi:10.1186/1471-2121-6-42