Analysis by SDS-PAGE of the cleavage of 125I-LPL in conditioned medium and the effect of an MMP inhibitor Explants of adipose tissue from fed rats were incubated as in Figure 1 for four hours and the medium was collected. 125I-LPL was then incubated at 4°C (to minimize the risk of conformational changes) in this medium with or without the MMP inhibitor GM6001 (5 μg/ml). Lane 1 – fresh medium, lane 2 – conditioned medium, lane 3 – conditioned medium + inhibitor. The lower band in lane 1 is a proteolytic fragment of LPL that is always present in preparations of the enzyme from bovine milk .
Wu et al. BMC Cell Biology 2005 6:4 doi:10.1186/1471-2121-6-4