LPL activity and mass within and outside the adipocytes. Conditions as in Figure 1 but adipose tissue explants from 180–200 g rats were used to get enough material to isolate adipocytes. At the end of the incubation some of the tissue explants were incubated with collagenase and adipocytes were isolated as described in the methods section. Filled symbols – fed rats; open symbols – fasted rats. Panel A shows LPL mass. ▲, △ – tissue, ▼, ▽ – adipocytes. Panel B shows LPL activity. ■, □ – tissue, ●, ○ – adipocytes. Values are means ± SEM for five parallel incubations. Some of the data points for fed and fasted rats fall on top of each other.
Wu et al. BMC Cell Biology 2005 6:4 doi:10.1186/1471-2121-6-4