HDAC1 and HDAC3 levels in proliferating and senescent WI-38 cells. Cytosolic and nuclear extracts were prepared from proliferating and senescent WI-38 cells. HDAC1 and HDAC3 levels were determined in both fractions by immunoblotting. Actin served as a loading control. The results from duplicate cultures are shown.
Place et al. BMC Cell Biology 2005 6:37 doi:10.1186/1471-2121-6-37